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Date of Publication: 2012-01-01

Journal: 中国药理学通报

Institution: 化工学院

Issue: 9

Page Number: 1218-1223

ISSN: 1001-1978

Abstract: Aim: To investigate the protective effects of protocatechuic acid (PCA) on cultured neural stem/progenitor cells (NS/PCs) differentiation. Methods: NS/PCs obtained from 14-day-old rat embryos were cultured under differentiation condition in the monolayer manner with or without PCA for the indicated time. Phenotypes differentiated from NS/PCs were visualized by immunofluorescent staining, the cell viability was determined by Cell Counting Kit-8 (CCK-8) test, and cell apoptosis was assayed by flow cytometric analysis. Results: PCA could promote the neuronal differentiation combined with fetal bovine serum (FBS) in vitro, although it could not initiate the differentiation of NS/PCs by itself. Moreover, PCA was able to induce neuronal maturation and efficiently promote neurite outgrowth. On the other hand, PCA facilitated the survival of phenotypes differentiated from cultured NS/PCs, which was associated with an increased percentage of the cellular viability and a decreased percentage of cells undergoing apoptosis under differentiation conditions. In addition, PCA-induced survival was also mediated with the activating of endogenous antioxidant enzymes. Conclusion: PCA promotes the neuronal differentiation and facilitates the survival of phenotypes differentiated from cultured NS/PCs.

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