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Date of Publication:2009-01-01

Journal:中国药理学通报

Affiliation of Author(s):化工学院

Issue:4

Page Number:448-452

ISSN No.:1001-1978

Abstract:Aim: To investigate the effects of PCA on cultured neural stem/progenitor cells (NS/PCs) proliferation and basal apoptosis. Methods: NS/PCs obtained from 14-day-old rat embryos were propagated as neurospheres and cultured under normal condition with or without PCA for the indicated time. The cell viability was determined by Cell Counting Kit-8 (CCK-8) test, while cell proliferation was assayed by bromodeoxyuridine ( BrdU) labeling. Results: After 4 days, basal apoptotic cells were detected and PCA significantly reduced the levels of apoptosis of NS/PCs. Treatment with 0.06 mmol��L-1 PCA caused 140.32% ��9.17% increase in cell viability compared with the control. Furthermore, on day 4 and day 7, the reactive oxygen species (ROS) level in NS/PCs was depressed by 43.8% �� 4.7% and 54.5% �� 6.1%, respectively, and caspase-3 activity was decreased by 70.6% �� 4.4% and 62.3% �� 5.5% respectively. Conclusions PCA can increase the cellular viability of NS/PCs and stimulate cell proliferation in a dose-and time-dependent manner.

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