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Date of Publication:2011-01-01

Journal:大连理工大学学报

Affiliation of Author(s):化工学院

Volume:51

Issue:5

Page Number:637-643

ISSN No.:1000-8608

Abstract:The collagen-chitosan composite scaffolds were fabricated by
   lyophilization technique, which were structurally modified by laminin
   (LN) protein, and their physical performance characteristics were
   determinated. Neural stem cells (NSCs) obtained from the fetal mouse
   hippocampus were cultured in the collagen-chitosan composite scaffolds
   and the biocompatibility was studied. The comparative results of
   different scaffolds with the parameters of aperture, porosity, water
   absorption and degradation rate show that the volume ratio of 7 : 3 is
   more suitable for cell culture in vitro and the inoculation rate of NSCs
   is markedly improved in LN-treated scaffolds. Through the observation of
   laser scanning confocal microscope (LSCM) and scanning electron
   microscopy (SEM), NSCs within collagen-chitosan composite scaffolds
   present good growth, proliferation and differentiation. These results
   provide a scientific experimental basis not only for further NSCs
   clinical transplantation applications, but also for the drug screening
   and mechanism research on the treatment of diseases of the nervous
   system.

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