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Indexed by:期刊论文

Date of Publication:2014-11-01

Journal:APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

Included Journals:SCIE、EI、PubMed、Scopus

Volume:174

Issue:6

Page Number:2114-2130

ISSN No.:0273-2289

Key Words:Neural stem cells (NSCs); In vitro culture; Collagen; Mathematical model

Abstract:The aim of this study is to analyze the growth and substance metabolism of neural stem cells (NSCs) cultured in biological collagen-based scaffolds. Mass transfer and metabolism model of glucose, lactic acid, and dissolved oxygen (DO) were established and solved on MATLAB platform to obtain the concentration distributions of DO, glucose, and lactic acid in culture system, respectively. Calculation results showed that the DO influenced their normal growth and metabolism of NSCs mostly in the in vitro culture within collagen-based scaffolds. This study also confirmed that 2-mm thickness of collagen scaffold was capable of in vitro cultivation and growth of NSCs with an inoculating density of 1 x 10(6) cells/mL.