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发表时间：2005-01-01

发表刊物：分析化学

卷号：33

期号：4

页面范围：501-504

ISSN号：0253-3820

摘要：A method using preparative high performance liquid chromatography ( HPLC) to isolate two individial alkaloids from cells of Camptotheca acuminata Decaisne was investigated. A preparative C-18 column was employed in the procedure. By isocratic elution with acetonitrile-water (35:65, V/V) as mobile phase at a flow rate of 5 mL/min, two alkaloids were obtained and subsequently were indetified by ultraviolet, infrared and mass spectroscopic methods as isocamptothecin A and isocamptothecin B. HPLC analytical results showed that the purity of each alkaloid was over 97%. Under the chromatographic conditions, every peak was completely baseline separated and consumption of solvents was low.

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