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个人信息Personal Information
副教授
硕士生导师
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：化学工程. 生物医学工程. 生物化工
办公地点：大连理工大学化工学院化工实验楼D413
联系方式：139玖捌伍肆捌柒壹柒
电子邮箱：guanshui@dlut.edu.cn
Mass Transfer Analysis of Growth and Substance Metabolism of NSCs Cultured in Collagen-Based Scaffold In Vitro
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论文类型：期刊论文
发表时间：2014-11-01
发表刊物：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
收录刊物：SCIE、EI、PubMed、Scopus
卷号：174
期号：6
页面范围：2114-2130
ISSN号：0273-2289
关键字：Neural stem cells (NSCs); In vitro culture; Collagen; Mathematical model
摘要：The aim of this study is to analyze the growth and substance metabolism of neural stem cells (NSCs) cultured in biological collagen-based scaffolds. Mass transfer and metabolism model of glucose, lactic acid, and dissolved oxygen (DO) were established and solved on MATLAB platform to obtain the concentration distributions of DO, glucose, and lactic acid in culture system, respectively. Calculation results showed that the DO influenced their normal growth and metabolism of NSCs mostly in the in vitro culture within collagen-based scaffolds. This study also confirmed that 2-mm thickness of collagen scaffold was capable of in vitro cultivation and growth of NSCs with an inoculating density of 1 x 10(6) cells/mL.