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论文类型：期刊论文

发表时间：2013-08-01

发表刊物：FOOD AND CHEMICAL TOXICOLOGY

收录刊物：SCIE、Scopus

卷号：58

页面范围：50-55

ISSN号：0278-6915

关键字：Catalpol; Aging; Cholinergic system; Inflammatory cytokines

摘要：The neuroprotective effects of catalpol, an iridoid glycoside isolated from the fresh rehmannia roots, on the cholinergic system and inflammatory cytokines in the senescent mice brain induced by D-galactose were assessed. The results showed that acetylcholinesterase (AChE) activity increased in senescent mice brain and choline acetyltransferase (ChAT) positive neurons, detected by immunohistochemical staining, decreased remarkably in the basal forebrain of senescent mice. Simultaneously, muscarinic acetylcholine receptor M1 (mAChR1) expression declined in senescent mice brain by western blotting method. We also found that the contents of tumor necrosis factor (THE-alpha), interleukin-1 beta (IL-1 beta) and advanced glycation endproducts (AGES) increased in senescent mice brain by ELISA method. However, administration of catalpol for 2-weeks significantly reversed the biochemical markers mentioned above. These results suggest that catalpol can exert protective effects on senescent mice brain induced by D-galactose and this effect may be due to its protective effects on cholinergic and immune impairment in mice brain. Thus catalpol is worth testing for further preclinical study aimed for senescence or neurodegenerative diseases such as Alzheimer's disease. (C) 2013 Elsevier Ltd. All rights reserved.