论文类型：期刊论文
发表刊物：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
收录刊物：PubMed、EI、SCIE
卷号：847
期号：2
页面范围：82-87
ISSN号：1570-0232
关键字：nitroaryl; nitroarene; arylhydroxylamine; aminoaryl; bakers' yeast; nitroarylreductase; HPLC; TLC
摘要：A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method was developed for the simultaneous determination of yield and conversionratio of 4-nitro-1,8-naphthalic anhydride to 4-amino-1,8-naphthalic: anhydride following incubation with a crude bakers' yeast homogenate. The analytes were separated on a C18 column in gradient mode. The detection limit of 4-amino-1,8-naphthalic anhydride is 10ng/mu l when using a 10 mu l sample injection volume. The nitroreductase activity in the homogenate system can be assessed during the bioconversion process. The method can be used for the simultaneous detection of 4-hydroxylamini)-1,8-naphthalic anhydride, an intermediate with limited stability. (c) 2006 Elsevier B.V. All rights reserved.