个人信息Personal Information
教授
博士生导师
硕士生导师
性别:男
毕业院校:日本冈山大学
学位:博士
所在单位:化工学院
学科:精细化工. 应用化学
办公地点:大连理工大学西部校区E209
联系方式:13591789942
电子邮箱:jncui@dlut.edu.cn
Real-Time Tracking the Synthesis and Degradation of Albumin in Complex Biological Systems with a near-Infrared Fluorescent Probe
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论文类型:期刊论文
发表时间:2017-09-19
发表刊物:ANALYTICAL CHEMISTRY
收录刊物:Scopus、SCIE、EI、PubMed
卷号:89
期号:18
页面范围:9884-9891
ISSN号:0003-2700
摘要:In this study, a novel fluorescent detection system for biological sensing of human albumin (HA) was developed on the basis of the pseudoesterase activity and substrate preference of HA. The designed near-infrared (NIR) fluorescent probe (DDAP) could be effectively hydrolyzed by HA, accompanied by significant changes in both color and fluorescence spectrum. The sensing mechanism was fully investigated by fluorescence spectroscopy, NMR, and mass spectra. DDAP exhibited excellent selectivity and sensitivity toward HA over a variety of human plasma proteins, hydrolases, and abundant biomolecules found in human body. The probe has been successfully applied to measure native HA in diluted plasma samples and the secreted HA in the hepatocyte culture supernatant. DDAP has also been used for fluorescence imaging of HA reabsorption in living renal cells, and the results show that the probe exhibits good cell permeability, low cytotoxicity and high imaging resolution. Furthermore, DDAP has been successfully used for real-time tracking the uptaking and degradation of albumin in ex vivo mouse kidney models for the first time. All these results clearly demonstrated that DDAP-based assay held great promise for real-time sensing and tracking HA in complex biological systems, which would be very useful for basic researches and clinical diagnosis of HA associated diseases.