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论文类型：期刊论文

发表时间：2008-07-01

发表刊物：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

收录刊物：SCIE、EI

卷号：24

期号：7

页面范围：1147-1152

ISSN号：0959-3993

关键字：Sphingomonas xenophaga; 1-aminoanthraquinone-2-sulfonic acid; anthraquinone dye intermediate; decolorization; degradation

摘要：Sphingomonas xenophaga QYY from sludge samples could effectively decolorize 1-aminoanthraquinone-2-sulfonic acid (ASA-2), one kind of anthraquinone dye intermediate, under aerobic conditions. More than 98% of ASA-2 could be removed within 120 h at the dye concentration from 200 mg l(-1) to 1,000 mg l(-1) due to oxidative degradation. The strain converted ASA-2 to 2-(2'-hydroxy-3'-amino-4'-sulfo-benzoyl)-benzoic acid, 2-(2'-amino-3'-sulfo-6'-hydroxy-benzoyl)-benzoic acid, o-phthalic acid and 2-amino-3-hydroxy-benzenesulfonic acid, which were identified using HPLC-MS and NMR. A possible initial decolorization pathway was proposed according to these metabolites. The decolorization of ASA-2 by cells in the basal salt medium was induced by ASA-2, and was due to soluble cytosolic enzymes. Combined initial decolorization pathway and the analysis of decolorization enzyme(s), the major enzyme responsible for ASA-2 decolorization was a NADH-dependent oxygenase.