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论文类型： 期刊论文

发表时间： 2014-03-01

发表刊物： MOLECULAR BIOLOGY REPORTS

收录刊物： SCIE、PubMed

卷号： 41

期号： 3

页面范围： 1879-1889

ISSN号： 0301-4851

关键字： MicroRNA; Phytophthora infestans; Late blight; miRNA-miRNA relationship network; Bioinformatics

摘要： Being one kind of approximately 22nt long small RNA, miRNA has shown its roles in host-pathogen interaction, providing one possible way for pathogen infection. Though Phytophthora infestans is a major pathogen that causes devastating late blight of potato, tomato and so on, so far there have not been any systematic researches on miRNAs and even pathogenic miRNAs in P. infestans. Here, for the first time we comprehensively predicted and identified pathogenic miRNAs that may exist in P. infestans. First, a total of 128 putative miRNAs belonging to 66 miRNA family were identified by bioinformatic approaches. Then, 33 vital pathogenic miRNAs were screened by constructing miRNA-miRNA relationship networks. Finally, four potential pathogenic miRNAs were chosen for detection, two of which are chosen for validation. The expression quantity of pi-miR466 and pi-miR1918 changed dramatically during incubation of tomato leaves, implying that they are potential pathogenic miRNAs.