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论文类型：期刊论文

发表时间：2013-07-01

发表刊物：MICROCHIMICA ACTA

收录刊物：Scopus、SCIE

卷号：180

期号：9-10

页面范围：829-835

ISSN号：0026-3672

关键字：Long-chain aptamer; Oxytetracycline; Fluorescent assay; Graphene

摘要：We report on a fluorescent assay for oxytetracycline (OTC) using a fluorescein-labeled long-chain aptamer assembled onto reduced graphene oxide (rGO). The pi-pi stacking interaction between aptamer and rGO causes the fluorescence of the label to be almost completely quenched via energy transfer so that the system has very low background fluorescence. The addition of OTC leads to the formation of G-quadruplex OTC complexes and prevents the adsorption of labeled aptamer on the surface of rGO. As a result, fluorescence is restored, and this effect allows for a quantitative assay of OTC over the 0.1-2 mu M concentration range and with a detection limit of 10 nM. This method is simple, rapid, selective and sensitive. It may be applied to other small molecule analytes by applying appropriate aptamers.