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Date of Publication:2010-01-01

Journal:大连理工大学学报

Affiliation of Author(s):环境学院

Issue:3

Page Number:340-345

ISSN No.:1000-8608

Abstract:A strain which can utilize phenol as sole carbon source is isolated from activated sludge of petrochemical wastewater and identified as Brucella sp. GXY-1 according to morphological, physiological and biochemical characteristics and 16S rDNA sequence analysis. The experimental results suggest that the strain can tolerate oxytetracycline and tetracycline. The optimum conditions for the degradation of phenol are temperture 30°C, pH 7.0. Under the optimum conditions, GXY-1 can decompose over 99% of phenol (600 mg/L) within 60 h. GXY-1 is able to degrade other aromatic compounds, such as aniline, naphthalene and chlorobenzene. Determination of enzymes activities in the degradation process indicates that the degradation of phenol is catalyzed by catechol 1, 2-dioxygenase. The optimum conditions for the enzymatic reaction are pH 7.8 and 40°C.

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