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Date of Publication:2013-01-01

Journal:大连理工大学学报

Affiliation of Author(s):海洋科学与技术学院

Issue:3

Page Number:346-353

ISSN No.:1000-8608

Abstract:The gene engineering strain PH_IND expressing phenol hydroxylase is used to catalyze the biotransformation of indole and methylindoles . The experimental results indicate that indole , 2-methylindole ,4-methylindole and 7-methylindole can be transformed into various indigoid pigments except for 3-methylindole . The predominant color products are identified by thin-layer chromatography and liquid chromatography-mass spectrometry analyses ,and the major products (blue pigment) derived from indole and 4-methylindole are authenticated as indigo and 4 ,4′-dimethylindigo , respectively .Meanwhile ,the biotransformation pathways of indole and 4-methylindole by PH_IND are proposed .Response surface methodology is applied to optimize the biotransformation conditions of indole ,2-methylindole and 4-methylindole by PH_IND .Under the optimal conditions ,the indigo yield and transformation efficiency of indole are 93 .74 mg/L and 52 .63% , respectively , while the maximum transformation rates of 2-methylindole and 4-methylindole are 67 .66% and 95 .61% , respectively .

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