个人信息Personal Information
副教授
博士生导师
硕士生导师
性别:男
毕业院校:大连理工大学
学位:博士
所在单位:生物工程学院
学科:生物化工
办公地点:生物工程学院547室
电子邮箱:renjun@dlut.edu.cn
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Overview of my current research work
In general, I am very interested in understanding the mechanism of biomolecule recognition, which plays central role in a variety of life activities, and hope to exploit its potential application in different area of biotechnology and biomedical applications.
(1) Biomedical materials for blood purification treatment
One specific interest is to develop techniques that enable recognizing and separating some specific disease-related molecules directly from human blood circulation, for achieving blood purification treatment for a range of serious diseases. To this end, we have developed several strategies to design functional materials, including affinity ligand screening, camelid-derived single chain antibody or nanobody, and molecular imprinting et al. These techniques are used to synthesize selective adsorbents for removing pathogenic factors from the blood of patients suffering from autoimmune diseases, renal failure, sepsis, and even cancer. For example, we are using nanobody to develop blood purification materials to treat dialysis related amyloidosis (DRA) by selectively removing beta 2 microglobulin. Meanwhile we are also isolating nanobodies against cytokines for synthesizing selective adsorbents, which could be used to control “Cytokine Storm” by directly reducing circulating cytokines from the blood of critically-ill patients with deadly inflammation.
Currently, two of my patented blood purification adsorbents have been industrialized to develop medical appliances for treating autoimmune disease and DRA, respectively. The former has been in the stage of clinical trial in China.
(2) Biomolecular engineering for bioprocessing and biohybrid materials
Other studies focus on engineering functional proteins and developing approaches for bioseparation, biosensing, and biofunctional device design, which are mainly based on the construction of biohybrid material system. Current work is centered at the application of engineering nanobody. The development of the methodologies involves production of engineered nanobody, specific tagging, multivalued complex, site-specific modification, bioconjugation, surface modification, as well as addressing issues such as stability, efficiency and activity rate in the process of applications.
We have been developing chemical and biological methods that could enable the immobilization of nanobody or other functional proteins in a controllable and efficient manner, to facilitate the preparation of affinity chromatography media, and biosensor surfaces. We used photochemical crosslinking of phenol groups to immobilize proteins and prepare all-protein nanoparticles. The reactions could be completed within seconds without loss of protein activity. Nanobody-based multiprotein complexes have been designed to form multifunctional protein assemblies.
These technologies and methods are expected to be extended to other biomedical areas, such as assembly of targeted nanoparticles for drug delivery, disease detection and mechanistic studies.
- Wang, Linghua, 赵明山, 韩秀友, 谷一英, Lv, Huanlin, Cheng, Jianfang, Teng, Jie, 任军, 王锦艳, 蹇锡高.Optical biosensors utilizing polymer-based athermal microring resonators[A],Conference on Biophotonics - Photonic Solutions for Better Health Care III,2022,8427
- Zhang, Lijun, Zang, Berlin, 黄春东, 任军, 贾凌云.One-step Preparation of a VHH-based Immunoadsorbent for the Extracorporeal Removal of 2-microglobulin[J],MOLECULES,2022,24(11)
- 任军, Wang, Duo, 贾凌云, 徐丽, 谢健.Study of hydrophobic interaction between immobilized ligands and proteins using an improved docking method[J],JOURNAL OF BIOTECHNOLOGY,2022,136:S484-S484
- Wei, Houliang, 任军, Han, Bo, 徐丽, 韩璐璐, 贾凌云.Stability of polydopamine and poly(DOPA) melanin-like films on the surface of polymer membranes under strongly acidic and alkaline conditions[J],COLLOIDS AND SURFACES B BIOINTERFACES,2022,110:22-28
- 任军, Yao, Peng, Chen, Jingjing, 贾凌云.Salt-independent hydrophobic displacement chromatography for antibody purification using cyclodextrin as supermolecular displacer[J],JOURNAL OF CHROMATOGRAPHY A,2022,1369:98-104
- 任军, 贾凌云, 徐丽, xuelin, Pi, Zhiqian, 谢健.Removal of autoantibodies by 4-mercaptoethylpyridine-based adsorbent[J],JOURNAL OF CHROMATOGRAPHY B ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND L,2022,877(11-12):1200-1204
- Comprehensive Biotechnology (3rd edition) 5.53 - Blood Detoxication
- Blood Detoxication
- Comprehensive Biotechnology, 2nd edition ,Volume 5: Medical Biotechnology and Healthcare/ Artificial Organs and Life Support Devices/ Blood Detoxication
- Regenerative Medicine, Artificial Cells and Nanomedicine: Volume 4
- 重金属稳定化空间演变机制, 国家科技部, 2022/11/20, 在研
- 以研究型能力培养为目标的创新实践课程建设(教育部产学合作协同育人), 企事业单位委托科技项目, 2021/09/06, 在研
- 除菌滤芯选择及工艺验证服务, 企事业单位委托科技项目, 2020/07/01, 在研
- 轮状病毒纳米抗体的筛选及亲和纯化介质的制备, 企事业单位委托科技项目, 2023/04/26, 在研
- 均一高强度、高载量、多模式琼脂糖介质的规模化制备和应用, 国家科技部, 2021/12/15, 在研
- 治疗疑难病症高效生物医学吸附材料共性关键技术研究及产业化示范, 省、市、自治区科技项目, 2021/07/14, 在研