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Indexed by:期刊论文

Date of Publication:2017-09-22

Journal:INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS

Included Journals:SCIE、EI、Scopus

Volume:66

Issue:14

Page Number:748-752

ISSN No.:0091-4037

Key Words:Cell adhesion; chemical cross-linking; copolymer; NIPAAm; temperature-sensitive

Abstract:A novel thermosensitive P (CS-g-TMSPM-g-NIPAAm) copolymer film was synthesized through grafting thermosensitive monomer N-isopropyl acrylamide (NIPAAm) onto the beforehand modified chitosan (CS) by cross-linking agent 3-trimethoxysilypropyl methacrylate (TMSPM). Fourier transform infrared spectroscopy confirmed the successful polymerization of NIPAAm with CS-g-TMSPM, and its lower critical solution temperature was measured at 34 degrees C. Although osteoblasts and adipose-derived stem cells showed excellent growth state on the film in vitro, only osteoblasts demonstrated good adhesion and stretch probably because of the necessary specific cell-film interplay. Hence, for specific cell cultures and nonenzymatic harvesting in vitro, the prepared thermosensitive film could be a promising approach.