Release Time:2019-03-13  Hits:

Indexed by: Journal Article

Date of Publication: 2016-10-01

Journal: ANIMAL CELLS AND SYSTEMS

Included Journals: Scopus、SCIE

Volume: 20

Issue: 5

Page Number: 276-281

ISSN: 1976-8354

Key Words: Bio-derived scaffold; osteoblast; fabrication; bioreactor; repair

Abstract: Fabrication and transplantation of tissue-engineered bones in a rotating wall vessel bioreactor (RWVB) was studied in the present study aiming to repair segmental bone defects. Osteoblasts were transfected with green fluorescent protein prior to seeding on bio-derived porous bone scaffolds at a density of 1x10(6)cells/mL and cultured in an RWVB for one week. For comparison, constructs were also cultured in a static condition. Morphology and structure of fabricated bones were examined using an inverted microscope, scanning electron microscope and histology analysis via hematoxylin-eosin and toluidine blue staining. Moreover, an animal model for repairing segmental bone defects of a Zelanian rabbit was used to assess the efficacy and biosafety of fabricated bones. In conclusion, tissue-engineered bones grew favorably in RWVB. In animal study, a preliminary repair of bone defects was noticed only in the experimental group after 4 weeks of implantation. Using RWVB, the fabricated tissue-engineered bone constructs were approved with better bio-capability in repairing the segmental bone defect.