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Indexed by:期刊论文

Date of Publication:2011-07-01

Journal:NEURAL REGENERATION RESEARCH

Included Journals:Scopus、SCIE

Volume:6

Issue:19

Page Number:1462-1466

ISSN No.:1673-5374

Key Words:cytosine deaminase gene; bone mesenchymal stem cells; glioma; transduction; migration

Abstract:This experiment sought to observe the migration and distribution of bone mesenchymal stem cells transfected with the cytosine deaminase gene (BMSCs-CD/eGFP) after transplantation in vivo through three pathways. In addition, we examined the tropism of these cells to glioma. Intracranial C6 glioma models were established in Sprague-Dawley rats using an intracranial stereotactic inoculation method. When tumors were 7 days old, rats were inoculated with 1x10(6) BMSCs-CD/eGFP cells via the tumor-bearing internal carotid artery, the contralateral hemisphere and the tumor-bearing glioma. Fluorescence microscopy revealed that BMSCs-CD/eGFP exhibited a strong capacity for migration to tumors. BMSCs-CD/eGFP transplanted via the tumor-bearing internal carotid artery were observed to distribute in glioma tissues. BMSCs-CD/eGFP inoculated via the ipsilateral glioma mainly located within and at the edge of glioma tissues. BMSCs-CD/eGFP inoculated via the contralateral hemisphere mainly distributed at the proximal end of the tumor at the incubation site.