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Indexed by:期刊论文

Date of Publication:2009-09-01

Journal:ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING

Included Journals:SCIE、EI、Scopus

Volume:4

Issue:5,SI

Page Number:765-770

ISSN No.:1932-2143

Key Words:mesenchymal stem cells; surface modification; biomaterials; AFM; morphology

Abstract:The Surface morphologies modified with chitosan and gelatin respectively, the effects oil cell spreading, adhesion and distribution of rabbit bone marrow-derived mesenchymal stem cells (rMSCs) were observed under atomic force microscope (AFM). The structures and morphologies of rMSCs on coverslips were qualitatively and quantitatively assayed under AFM. Thereafter, the growth status and viability of rMSCs on the different coated surfaces were examined under inverted microscope and by Hoechst/P1 double staining. The results showed that the surfaces modified with different materials presented different morphologies. The rMSCs adhered to the surfaces modified with 2 mg/ml chitosan and 0.5 mg/ml gelatin solution showed better viabilities than those of other groups, and the latter also presented the optimal cell adhesion, spreading and distribution with clear cellular structure such as cell nucleus. villus and interaction among various rMSCs. It is indicated that the surface modification played an important role on the development of cellular structure and function of rMSCs, and thus provided theoretical guidance to the fabrication and surface modification of embedded biomaterials in further clinical applications. (c) 2009 Curtin University of Technology and John Wiley & Soils, Ltd.