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BMMSCs在温敏性玻璃微载体上的培养及收获

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Indexed by:期刊论文

Date of Publication:2016-06-15

Journal:高校化学工程学报

Included Journals:EI、PKU、ISTIC、CSCD

Volume:30

Issue:3

Page Number:633-640

ISSN No.:1003-9015

Key Words:N-异丙基丙烯酰胺;温敏性玻璃微载体;表面自由基共聚合;骨髓间充质干细胞;降温脱附

Abstract:贴壁型种子细胞的培养和收获,常在二维静态的培养环境下进行,而后采用酶解法或机械刮刀法来进行收获,但是其存在着一定的弊端,细胞扩增数量有限且在收获时细胞易受到损伤,同时也会失去一定的干性.论文研究整合了具有较大比表面积和三维立体环境的微载体,及温敏性材料无损收获细胞的优点,采用表面自由基共聚合方法,用3-丙基三甲氧基硅烷甲基丙烯酸TMSPM为偶联剂,在微载体表面接枝上N-异丙基丙烯酰胺NIPAAm(N-isopropylacrylamide)及甲基丙烯酸羟基丙基酯(Hydroxypropyl methacrylate,HPM), 制备出P(NIPAAm-co-HPM)-g-TMSPM-g-徼载体.其次,将骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMMSCs)接种在制备的温敏性玻璃微载体上,通过细胞的培养与降温收获考察制备的温敏性微载体是否适宜细胞的降温脱附及其细胞相容性.扫描电镜及能谱分析表明温敏材料NIPAAm可成功地接枝到含硅羟基的玻璃微载体表面.BMMSCs的粘附和生长曲线测定结果证明材料具有较好的生物相容性,降温收获后细胞的活性对比及再培养实验表明细胞可很好的从载体上脱附,并保持良好的生长活性.

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