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论文类型：期刊论文

发表时间：2014-05-01

发表刊物：BIOTECHNOLOGY LETTERS

收录刊物：SCIE、EI

卷号：36

期号：5

页面范围：947-960

ISSN号：0141-5492

关键字：Bioreactors; Cardiac tissue engineering; Embryonic stem cells; Stem cell differentiation; Three-dimensional culture; Tissue engineering; Troponin

摘要：Developments in bioprocessing technology play an important role for overcoming challenges in cardiac tissue engineering. To this end, our laboratory has developed a novel rotary perfused bioreactor for supporting three-dimensional cardiac tissue engineering. The dynamic culture environments provided by our novel perfused rotary bioreactor and/or the high-aspect rotating vessel produced constructs with higher viability and significantly higher cell numbers (up to 4 x 10(5) cells/bead) than static tissue culture flasks. Furthermore, cells in the perfused rotary bioreactor showed earlier gene expressions of cardiac troponin-T, alpha- and beta-myosin heavy chains with higher percentages of cardiac troponin-I-positive cells and better uniformity of sacromeric alpha-actinin expression. A dynamic and perfused environment, as provided by this bioreactor, provides a superior culture performance in cardiac differentiation for embryonic stem cells particularly for larger 3D constructs.