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发布时间：2019-03-12

论文类型：期刊论文

发表时间：2017-09-22

发表刊物：INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS

收录刊物：Scopus、EI、SCIE

卷号：66

期号：14

页面范围：748-752

ISSN号：0091-4037

关键字：Cell adhesion; chemical cross-linking; copolymer; NIPAAm; temperature-sensitive

摘要：A novel thermosensitive P (CS-g-TMSPM-g-NIPAAm) copolymer film was synthesized through grafting thermosensitive monomer N-isopropyl acrylamide (NIPAAm) onto the beforehand modified chitosan (CS) by cross-linking agent 3-trimethoxysilypropyl methacrylate (TMSPM). Fourier transform infrared spectroscopy confirmed the successful polymerization of NIPAAm with CS-g-TMSPM, and its lower critical solution temperature was measured at 34 degrees C. Although osteoblasts and adipose-derived stem cells showed excellent growth state on the film in vitro, only osteoblasts demonstrated good adhesion and stretch probably because of the necessary specific cell-film interplay. Hence, for specific cell cultures and nonenzymatic harvesting in vitro, the prepared thermosensitive film could be a promising approach.