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论文类型：期刊论文

发表时间：2013-10-04

发表刊物：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录刊物：SCIE、PubMed、Scopus

卷号：439

期号：4

页面范围：552-558

ISSN号：0006-291X

关键字：Polycistronic plasmid; Human adipose-derived stem cells; Neural differentiation

摘要：Somatic cell reprogramming may become a powerful approach to generate specific human cell types for cell-fate determination studies and potential transplantation therapies of neurological diseases. Here we report a reprogramming methodology with which human adipose stem cells (hADSCs) can be differentiated into neural cells. After being reprogrammed with polycistronic plasmid carrying defined factor OCT3/4, SOX2, KLF4 and c-MYC, and further treated with neural induce medium, the hADSCs switched to differentiate toward neural cell lineages. The generated cells had normal karyotypes and exogenous vector sequences were not inserted in the genomes. Therefore, this cell lineage conversion methodology bypasses the risk of mutation and gene instability, and provides a novel strategy to obtain patient-specific neural cells for basic research and therapeutic application. (C) 2013 Elsevier Inc. All rights reserved.