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论文类型：期刊论文

发表时间：2012-11-06

发表刊物：ENVIRONMENTAL SCIENCE & TECHNOLOGY

收录刊物：SCIE、EI、PubMed、PKU、ISTIC、Scopus

卷号：46

期号：21

页面范围：12170-12177

ISSN号：0013-936X

摘要：Proteomic analysis allows detection of changes of proteins expression in organisms exposed to environmental pollutants, leading to the discovery of biomarkers of exposure and understanding of the action mechanism of toxicity. In the present study, we applied iTRAQ labeling quantitative proteomic technology for global characterization of the liver proteome in mice exposed to perfluorooctane sulfonate (PFOS). This successfully identified and quantified 1038 unique proteins. Seventy-one proteins showed a significant expression change in the treated groups (1.0, 2.5, 5.0 mg/kg of body weight) compared with the control group, and 16 proteins displayed strong dose dependent changes. Gene ontology analysis showed that these differential proteins were significantly enriched and stimulus. We detected significantly increased expression levels of enzymes regulating mainly involved in lipid metabolism, transport, biosynthetic processes, and response to peroxisomal beta-oxidation-including long chain acyl-CoA synthetase, acyl-CoA oxidase 1, bifunctional enzyme, and 3-ketoacyl-CoA thiolase A. PFOS also significantly induced cytochrome P450s and glutathione S-transferases that are responsible for the metabolism of xenobiotic compounds. The expressions of several proteins with important biological functions-such as cysteine sulfinic acid decarboxylase, aldehyde dehydrogenase, and apolipoprotein A-I, also correlated with PFOS exposure. Together, the present results provide insight into the molecular mechanism and biomarkers for PFOS-induced effects.