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论文类型： 期刊论文

发表时间： 2017-09-05

发表刊物： CHEMBIOCHEM

收录刊物： Scopus、SCIE、PubMed

卷号： 18

期号： 17

页面范围： 1762-1769

ISSN号： 1439-4227

关键字： dyes; pigments; fluorescence; proteins; SNAP-tag; two-photon imaging

摘要： Genetically encoded technologies provide methods for the specific labeling and imaging of proteins, which is essential to understand the subcellular localization of these proteins and their function. Herein, we employed naphthalimide, an efficient two-photon fluorophore, to develop O-6-benzylguanine (BG) derivatives for specific labeling of subcellular proteins and fluorescent imaging through the SNAP-tag. Three naphthalimide-BG derivatives, TNI-BG, QNI-BG, and ONI-BG, were conveniently synthesized through modular click chemistry in high yields. All of them showed high labeling efficiency with SNAP-tag in solution (approximate to 1-2x10(3)s(-1)m(-1)) and in bacteria. Among them, ONI-BG showed high specificity to diffused, histone H2B and mitochondria COX8A targeted SNAP-tag in mammalian cells. The protein-labeled naphthalimides exhibited high two-photon absorption cross-sections, which indicated their potential application in protein-specific two-photon fluorescent imaging, such as two-photon fluorescent lifetime imaging and two-photon multicolor imaging. Therefore, ONI-BG is a versatile tool that can be used to track subcellular proteins through multiple fluorescent techniques.