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Indexed by:Journal Papers

Date of Publication:2020-01-22

Journal:Biosensors & bioelectronics

Included Journals:EI、PubMed

Volume:154

Page Number:112039

ISSN No.:1873-4235

Key Words:Au NPs,Fiber-optic SPR,Heparin,Label-free detection,Serum

Abstract:A label-free Au NPs-enhanced surface plasmon resonance (SPR) sensor was developed for the ultrasensitive detection of heparin based on competitive adsorption behavior of heparin and Au NPs on the poly (dimethyl-diallylammonium chloride) (PDDA)-modified optical fiber surface and the corresponding change in the resonance wavelength of SPR. Due to the high affinity between heparin and PDDA, the present senor shows good analytical performance with respect to heparin detection. Two obvious advantages of the proposed heparin sensor over other reported methods are: its much wider linear concentration range (10-6-10-10 g/mL) and lower limit of detection (0.0257 ng/mL). The analysis of heparin in serum demonstrated that the present sensor exhibited high sensitivity and selectivity. It should be noted that the sensing strategy takes advantage of a portable fiber-optic SPR sensing system and avoids the need for complex processes for labeled-Au NPs, and thus the present sensor promises to be a practical tool for the point-of-care monitoring of heparin.Copyright © 2020 Elsevier B.V. All rights reserved.