研究方向：

功能染料的设计开发与在生物、医学、信息领域的应用研究。

1）肿瘤标志物识别荧光染料探针的设计开发，旨在通过快速、准确检测标志物代谢水平，探究标志物间相互作用，发掘荧光染料探针在癌症早期诊断、发病机理研究方面的生物医学应用。

2）肿瘤靶向荧光导航手术染料试剂设计开发。

3）器官靶向脂质纳米药物/疫苗设计开发。

专利申请：

11、张长玉,徐子崴,李建卫,杜健军,樊江莉。一类硝基咪唑基团中位取代的七甲川菁染料、其制备方法与应用，发明专利，已受理，2025117320698。

10、吴伟主,张长玉,徐子崴,吴旻骅,刘元萌,樊江莉。一种靶向人表皮生长因子受体2 的荧光染料及制备方法与应用，发明专利，已受理，2026100352427。

9、张长玉,刘元萌,马婉莹,徐子崴,李建卫,孙文,杜健军,樊江莉。一类人胱硫醚γ-裂解酶响应荧光染料及其制备方法与应用，发明专利，已授权，ZL 2024 1 1673322.2。

8、樊江莉,吴冰冰,夏祥,张长玉,杜健军,彭孝军。一种基于吩噻嗪细胞膜靶向光敏剂及其制备方法和应用。发明专利，已受理，2025102844934。

7、樊江莉,顾华,孙文,张长玉,杜健军,彭孝军。非共轭N-甲基位点连接的光敏染料及其制备方法与应用。发明专利，已受理，2023117217255。

6、樊江莉,李子鹏,张长玉,彭孝军。一类三甲川吡啶菁染料、其制备方法和应用。发明专利，已授权，ZL 2024 1 0141492.X。

5、张长玉，吴伟主，马婉莹，刘佳，刘钲，顾华，孙文，樊江莉。一种KIAA1363 酶靶向近红外荧光染料及其制备方法与应用，发明专利，已授权，ZL202311592186X。

4、樊江莉,顾华,孙文,张长玉,杜健军,彭孝军。非共轭N-甲基位点连接的光敏染料及其制备方法与应用，发明专利，已受理，202311721725.5。

3、樊江莉,张长玉,刘钲,孙文,杜健军,彭孝军。一种靶向内质网的大斯托克斯位移荧光染料及其制备方法与应用，发明专利，已授权，ZL 2024 1 0713821.3。

2、樊江莉,李常乐,张长玉,孙文,杜健军,彭孝军。一类雌激素受体阳性乳腺癌荧光探针、制备方法与应用，发明专利，已受理，202410161649.5。

1、孙文，苏泽厚，张长玉，樊江莉，彭孝军。一种ATP 响应的用于肿瘤化疗与光动力联合治疗的纳米药物及其制备方法，发明专利，已授权，ZL202211087677.4。

论文发表：

25、Changyu Zhang*, Ziwei Xu, Weizhu Wu, Yuanmeng Liu, Jianwei Li, Wen Sun, Jianjun Du, Jiangli Fan*, and Xiaojun Peng. Precision Delineation of Surgical Margins across Breast Cancer Subtypes with a Novel KIAA1363-Targeted NIR Fluorescent Probe. ACS Sens. 2026, DOI: 10.1021/acssensors.5c04472.

24、Changyu Zhang*, Yuanmeng Liu,  Weizhu Wu, Longjin Liu, Jiaxi Ru, Jianwei Li, Wen Sun, Jianjun Du, Jiangli Fan*, and Xiaojun Peng. The First NIR Fluorescent Probe for Bioimaging of Cystathionine-γ-Lyase Activity in Living Cells and Xenograft Tumor Models. Anal. Chem. 2026, 98,  6392.

23、Zheng Liu, Changyu Zhang*, Zipeng Li, Wanying Ma, Jia Liu, Xiang Xia, Ning Xu,Wen Sun, Jianjun Du, Jiangli Fan1*, Xiaojun Peng. Visualization of the protective role of estrogen against female liver fibrosis via an er viscosity NIR fluorescent probe. Sci. China Chem. 2025, 68, 360-368.

22、Haosheng Shen, Changyu Zhang, Shengjie Li, Yuanmei Liang, Li Ting Lee, Nikhil Aggarwal, Kwok Soon Wun, Jing Liu, Saravanan Prabhu Nadarajan, Cheng Weng, Hua Ling, Joshua K. Tay, De Yun Wang, Shao Q. Yao, In Young Hwang, Yung Seng Lee, Matthew Wook Chang. Nat Commun. 2024, 15, 4343.

21、Changle Li, Changyu Zhang,* Wenkai Liu, Jia Liu, Wanying Ma, Chengyuan Lv, Zhuoran Xia, Yingchao Chen, Hua Gu,Wen Sun, Jianjun Du, Jiangli Fan,* Xiaojun Peng. Clearly fluorescent delineating ER+ breast tumor incisal edge and identifying tiny metastatic tumor foci at high resolution. J. Mater. Chem. B. 2024, 12, 7135-7142.

20、XiaoDong, SingYee Ong,Changyu Zhang,Wenqiang Chen, Shubo Du,Qicai Xiao,Liqian Gao,and Shao Q.Yao. ACS Sens. 2021, 6, 3102-3111.

19、C. Zhang, X Dong, S. Y. Ong, S. Q. Yao. Live-cell imaging of survivin mRNA by using a dual-color surface-crosslinked nanoquencher. Anal. Chem. 2021, 93, 12081–12089.

18、S. Y. Ong,‡ C. Zhang,‡ X. Dong, S. Q. Yao. Recent advances in polymeric nanoparticles for enhanced fluorescence and photoacoustic imaging. Angew. Chem. Int. Ed. 2021, 60, 17797-809.

17、 C. Zhang,‡ Q.-Z. Zhang,‡ K. Zhang,‡ L.-Y. Li, M. D. Pluth; L. Yi, Z. Xi. Dual-biomarker-triggered fluorescence probes for differentiating cancer cells and revealing synergistic antioxidant effects under oxidative stress. Chem. Sci. 2019, 10, 1945-1952. (inside front cover).

16、Y. Xie, C. Zhang, Z. Wang, C. Wei, N. Liao, X. Wen, C. Niu, L. Yi, Z. Wang, Z. Xi. Fluorogenic assay for acetohydroxyacid synthase: design and applications. Anal. Chem. 2019, 91, 13582-90.

15、C. Zhang,‡ Q. Nie,‡ I. Ismail, Z. Xi, L. Yi. A highly sensitive and selective fluorescent probe for fast sensing of endogenous HClO in living cells. Chem. Commun. 2018, 54, 3835-3838.

14、C. Zhang,‡ L. Wei,‡ C. Wei, J. Zhang, R. Wang, Z. Xi, L. Yi. A FRET-ICT dual-quenching fluorescent probe with large off-on response for H2S: synthesis, spectra and bioimaging. Chem. Commun. 2015, 51, 7505-8.

13、H. Zhang,‡ C. Zhang,‡ R. Liu, L. Yi, H. Sun. A highly selective and sensitive fluorescent thiol probe through dual-reactive and dual-quenching groups. Chem. Commun. 2015, 51, 2029-32. (back cover).

12、C. Zhang, R. Wang, L. Cheng, B. Li, Z. Xi, L. Yi. A redox-nucleophilic dual-reactable probe for highly selective and sensitive detection of H2S: synthesis, spectra and bioimaging. Sci. Rep. 2016, 6: 30148.

11、C. Zhang,‡ B.-B. Zhai,‡ T. Peng, Z. Zhong, L. Xu, Q.-Z. Zhang, L.-Y. Li, L. Yi, Z. Xi. Design and synthesis of near-infrared fluorescence-enhancement probes for the cancer-specific enzyme hNQO1. Dyes Pigm. 2017, 143, 245-251.

10、 J. Li,‡ C. Zhang,‡ S. Wu, X. Wen, Z. Xi, L. Yi. Facile synthesis of green-light and large Stokes-shift emitting coumarins for bioconjugation. Dyes Pigm. 2018, 151, 303-9.

9、 C. Zhang, S. Wu, Z. Xi, L. Yi. Design and synthesis of NBD-S-dye dyads for fluorescently discriminative detection of biothiols and Cys/Hcy. Tetrahedron. 2017, 73, 6651-6.

8、Y. Huang,‡ C. Zhang,‡ Z. Xi, L. Yi. Synthesis and characterizations of a highly sensitive and selective fluorescent probe for hydrogen sulfide. Tetrahedron Lett. 2016, 57, 1187-91.

7、 H. Huang, X. Ji, Y. Jiang, C. Zhang, X. Kang, J. Zhu, L. Sun, L. Yi. NBD-based fluorescent probes for separate detection of cysteine and biothiols via different reactivities. Org. Biomol. Chem., 2020, 18, 4004-08.

6、I. Ismail, D. Wang, Z. Wang, D. Wang, C. Zhang, L. Yi, Z. Xi. A julolidine-fused coumarin-NBD dyad for highly selective and sensitive detection of H2S in biological samples. Dyes Pigm. 2019, 163, 700-6.

5、 Y. Jiang, X. Ji, C. Zhang, Z. Xi, L. Sun, L. Yi. Dual-quenching NBD-based fluorescent probes for separate detection of H2S and Cys/Hcy in living cells. Org. Biomol. Chem., 2019, 17, 8435-42.

4、 D. Ma, J. Zhang, C. Zhang, Y. Men, H. Sun, L.-Y. Li, L. Yi, Z. Xi. A highly efficient dual-diazonium reagent for protein crosslinking and construction of a virus-based gel. Org. Biomol. Chem., 2018, 16, 3353-57.

3、 R. Wang, Z. Li, C. Zhang, Y. Li, G. Xu, Q.-Z. Zhang, L.-Y. Li, L. Yi, Z. Xi. Fast‐response turn‐on fluorescent probes based on thiolysis of NBD amine for H2S bioimaging. ChemBioChem. 2016, 17, 962-8.

2、C. Wei, R. Wang, C. Zhang, G. Xu, Y. Li, Q.-Z. Zhang, L.-Y. Li, L. Yi, Z. Xi. Dual-reactable fluorescent probes for highly selective and sensitive detection of biological H2S. Chem. Asian J. 2016, 11, 1376-81.

1、 L. Yi, L. Wei, R. Wang, C. Zhang, J. Zhang, T. Tan, Z. Xi. A dual-response fluorescent probe reveals the H2O2-induced H2S biogenesis through a cystathionine β-synthase pathway. Chem. Eur. J. 2015, 21, 15167-72.