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论文类型：期刊论文

发表时间：2017-12-01

发表刊物：BIORESOURCE TECHNOLOGY

收录刊物：Scopus、SCIE、EI、PubMed

卷号：245

期号：,SI

页面范围：1603-1609

ISSN号：0960-8524

关键字：L-Glutamine; L-Alanyl-L-glutamine; alpha-Amino acid ester acyltransferase; Sphingobacterium siyangensis; Repeated-cycle

摘要：In this study, the optimum induction and reaction conditions, and fermentation process of producing Ala-Gln by E. coli Origami 2 overexpressing alpha-amino acid ester acyltransferase (OPA) were investigated. Besides, the Ala-Gln synthesis by OPA achieved the maximum molar yield of 94.7% and productivity of 1.89 g/L/min due to the extremely high enzyme activity. On this basis, repeated-cycle batch fermentation to produce Ala-Gln indicated that OPA could maintain high Ala-Gln yields and enzyme stabilities after several cell recycling. Consequently, the cost-efficient and environmentally friendly approach for Ala-Gln production by recycling OPA makes a great contribution to further industrial-scale applications. (C) 2017 Published by Elsevier Ltd.