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Indexed by:期刊论文

Date of Publication:2021-03-05

Journal:INDUSTRIAL CROPS AND PRODUCTS

Volume:158

ISSN No.:0926-6690

Key Words:Salvia miltiorrhiza Bunge; Phenolics; Extraction; Enrichment; HPLC-DAD analysis

Abstract:Salvia miltiorrhiza Bunge leaves, a crop waste, have potential as a source of bioactive phenolics. In this work, ultrasound-assisted extraction (UAE) was employed as a green extraction technology to extract phenolics from S. miltiorrhiza leaves. UAE parameters were optimized using response surface methodology (RSM) based on BoxBehnken design, achieving the total phenolics from S. miltiorrhiza leaves (SMLTP) yield of 71.16 +/- 0.57 mg gallic acid equivalent per g of dry material (mg GAE/g DM) under optimal conditions. Afterwards, NKA-2 resin was selected to enrich SMLTP, the data for adsorption of SMLTP on NKA-2 resin fitted well with pseudo-second-order and Langmuir models. A NKA-2 resin-based chromatography procedure was optimized by studying breakthrough curves and desorption curves to enrich SMLTP from crude extract. A lab-scale enrichment experiment was performed under optimal conditions, the content of SMLTP in the product was 78.33 +/- 0.61 %, which was 3.7 times higher than that in the crude extract, the recovery yield of SMLTP was 82.14 +/- 1.62 %. Finally, HPLC-DAD analysis showed the contents of five major phenolic compounds including caffeic acid, rutin, isoquercitrin, rosmarinic acid, and salvianolic acid B significantly increased through enrichment. Certainly, this study will enhance S. miltiorrhiza industry to be more ecologic and economic.