Release Time:2022-06-21  Hits:

Date of Publication: 2011-01-01

Journal: 过程工程学报

Issue: 6

Page Number: 1030-1037

ISSN: 1009-606X

Abstract: Based on the complex treatment of plasma and LiCl, a mutant strain of Klebsiella pneumoniae was isolated by using the medium containing FeCl 3 and selective pressure. The mutant strain named KpM30 improved 1,3-propanediol (1,3-PD) production from biodiesel-derived glycerol through producing less organic acid than the wild strain. The fed-batch fermentation in a 5 L bioreactor showed that the production of 1,3-PD reached 70.2 g/L, its yield on glycerol was 0.576 (mol/mol), which were increased by 24.9% and 17.7% compared with the wild strain. Meanwhile, the production of lactic acid was decreased by 47.0%. According to the metabolic analysis, the production of ATP and NADH 2 by KpM30 were 1.6-fold and 1.3-fold of the wild strain at the beginning of the fermentation. At the end of the fermentation, the carbon ?ux ?owed from glycerol to 1,3-PD was increased by 22.6%. For the less production of organic acid and better use of biodiesel-derived glycerol, this mutated strain was helpful for the separation process after fermentation and the integrated bioprocess combining biodiesel with 1,3-PD production.

Note: 新增回溯数据