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发酵条件对产2,3-T二醇Klebsiella pneumoniae代谢的影响

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Date of Publication:2022-10-09

Journal:食品与发酵工业

Affiliation of Author(s):海洋科学与技术学院

Volume:36

Issue:8

Page Number:6-11

ISSN No.:0253-990X

Abstract:The batch fermentation for 2,3-butanediol production by Klebsiella
   pneumon, iae CICC 10011 was investigated. Theresultsshowedt
   hattheoptimumconditionsfo rfermentationwere 0.10 vvm of airflow,5.8 of
   pHvalue. The concentration of target products (2,3-butanediol and
   acetoin)was 15.76 g/L with a productivity of l.97 g/(L. h)after 8 hours'
   batch fermentation under the optimized conditions, corresponding to 32%
   of yield from glucose. The glucose batch fermentation by Klebsiella
   pneumoniae CICC 10011 exhibited the sequential synthesis of main
   products, and acetate formation was earlier than other products
   formation. Based on the analysis of cell growth and formation of main
   products, the early formation of acetate is favorable for 2,3-butanediol
   production. Therefore,it is important to produce adequate acetate as
   early as possible and maintain long logarithmic growth phase by means of
   adjusting substrate concentration, air flow and pH

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