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Date of Publication:2022-10-09
Journal:食品与发酵工业
Affiliation of Author(s):海洋科学与技术学院
Volume:36
Issue:8
Page Number:6-11
ISSN No.:0253-990X
Abstract:The batch fermentation for 2,3-butanediol production by Klebsiella
pneumon, iae CICC 10011 was investigated. Theresultsshowedt
hattheoptimumconditionsfo rfermentationwere 0.10 vvm of airflow,5.8 of
pHvalue. The concentration of target products (2,3-butanediol and
acetoin)was 15.76 g/L with a productivity of l.97 g/(L. h)after 8 hours'
batch fermentation under the optimized conditions, corresponding to 32%
of yield from glucose. The glucose batch fermentation by Klebsiella
pneumoniae CICC 10011 exhibited the sequential synthesis of main
products, and acetate formation was earlier than other products
formation. Based on the analysis of cell growth and formation of main
products, the early formation of acetate is favorable for 2,3-butanediol
production. Therefore,it is important to produce adequate acetate as
early as possible and maintain long logarithmic growth phase by means of
adjusting substrate concentration, air flow and pH
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