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Indexed by:期刊论文

Date of Publication:2019-05-05

Journal:SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

Included Journals:SCIE、PubMed、EI

Volume:214

Page Number:469-475

ISSN No.:1386-1425

Key Words:Fluorescent probe; Cysteine detection; Large Stokes shift; Fluorescence imaging

Abstract:Fluorescent probes with high quality for highly selective detection of cysteine (Cys) are still urgently in demand because of the indispensable roles Cys plays in the biological systems. Herein, a red-emitting fluorescent probe CP was developed for the highly selective detection of Cys over glutathione (GSH) and homocysteine (Hcy) by incorporating acryloyl group as the recognition unit into the 2-(2-(4-hydroxystyryl)-6-methyl-4H-pyran-4-ylidene)malononitrile (P-OH) fluorophorewhich is characterized by redemission, noteworthy Stokes shift, and appreciable photostability. Basically, CP demonstrated appreciable sensing performance toward Cys including short response time of 4 min, high sensitivity with approximately 147-fold emission enhancement, low detection limit of 41.696 nM, and good selectivity both in the solution and living cells, indicating its promising potential of visualizing Cys in biological systems. (C) 2019 Elsevier B.V. All rights reserved.