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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
Quantitative recognition and ratiometric cell imaging of HSO3- inspired of confined-space based FRET system within human serum albumin
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论文类型：期刊论文
发表时间：2018-08-15
发表刊物：SENSORS AND ACTUATORS B-CHEMICAL
收录刊物：SCIE、EI
卷号：267
页面范围：104-110
ISSN号：0925-4005
关键字：Fluorescent probe; Ratiometric imaging; Confined-space mechanism; Sulfite determination; FRET fabrication
摘要：A ratiometric fluorescent probe for HSO3- was assembled using a pair of Forster resonance energy transfer (FRET)-matchable fluorescent dyes within a single human serum albumin (HSA). In this work, the micro environment-sensitive dyes B1 and HE were designed and synthesized, wherein B1 located in site II as the FRET donor while HE located in site I as the FRET acceptor and receptor for HSO3-. This B1-HE FRET system exhibited a good efficiency in the sensitive, selective, and ratiometric recognition of HSO3- with a detection limit of 99 nM in aqueous media. Furthermore, this B1-HE system could realize the ratiometric imaging of HSO3- in human breast cancer cells. (C) 2018 Elsevier B.V. All rights reserved.