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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
A two-photon NIR-to-NIR fluorescent probe for imaging hydrogen peroxide in living cells
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论文类型：期刊论文
发表时间：2017-08-15
发表刊物：BIOSENSORS & BIOELECTRONICS
收录刊物：SCIE、EI、PubMed、Scopus
卷号：94
页面范围：536-543
ISSN号：0956-5663
关键字：Two-photon; NIR emission; Ultra-low detection limit; Hydrogen peroxide; Cell imaging
摘要：Hydrogen peroxide (H2O2), one of the reactive oxygen species (ROS), plays vital roles in diverse physiological processes. Imbalance of the H2O2 is concerned with serious diseases such as cardiovascular disorders, neurodegenerative diseases, Alzheimer's disease and cancer. Therefore, it is critical to develop efficient methods for monitoring H2O2 in vivo. In this work, a two-photon excitation (860 nm) NIR fluorescent turn-on probe TPNR-H2O2 for H2O2 based on Dicyanomethylene-4H-pyran fluorophore is reported, which can be used in solution detection with 13.2-fold NIR fluorescence enhancement, fast response (completed within 40 min), excellent sensitivity (DL 72.48 nM), and lower cellular auto-fluorescence interference. Importantly, the perfect photostability of TPNR-H2O2 clearly demonstrated that the probe could be applied to imaging intracellular H2O2 for a long time without photobleaching. In addition, through two-photon imaging, this probe was cell permeable and used to monitor the level of endogenous and exogenous H2O2 with promising biological application.