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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
Fluorescence completely separated ratiometric probe for HClO in lysosomes
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论文类型：期刊论文
发表时间：2017-07-01
发表刊物：SENSORS AND ACTUATORS B-CHEMICAL
收录刊物：SCIE、EI、Scopus
卷号：246
页面范围：293-299
ISSN号：0925-4005
关键字：Hypochlorous acid; Ratiometric probe; Completely separated fluorescent signals; Lysosomes
摘要：Hypochlorous acid (HClO), as one of the reactive oxygen species (ROS), was reported to be involved in a number of human diseases and many efforts have therefore been made to probe its biological roles at molecular level. In this work, a ratiometric probe (BFCIO) with completely separated fluorescent signals consisting of a boron-dipyrromethene (BODIPY) dye conjugated with Fisher aldehyde.was designed and synthesized for the selective and sensitive detection of HOCI via oxidative cleavage of the alkene linker. The probe evinced more than 300-fold enhancement in fluorescence ratio(I-511nm/I-713nm) in the presence of HClO over other ROS with fast response time (within 10 s) and low detection limit (10.6 nM). Confocal fluorescence images demonstrated that the probe could well permeate through plasma membrane and visualize exogenous and endogenous HOCI in lysosomes of living cells. (C) 2017 Elsevier B.V. All rights reserved.