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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：Director, State Key laboratory of Fine Chemicals
其他任职：精细化工国家重点实验室主任、国务院学科评议组成员
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学精细化工国家重点实验室
西部校区化工实验楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学精细化工国家重点实验室 西部校区化工实验楼F-202 辽宁省大连市高新区凌工路2号，大连116024 Tel: 0411-84986306; Fax: 0411-84986292；课题组网址：http://peng-group.dlut.edu.cn/
电子邮箱：pengxj@dlut.edu.cn
A facile ratiometric sensing platform based on inner filter effect for hypochlorous acid detection
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论文类型：期刊论文
发表时间：2021-04-12
发表刊物：SENSORS AND ACTUATORS B-CHEMICAL
卷号：325
关键字：HOCl detection; Ratiometric sensing platform; Inner filter effect; Weak cross-talk; Cell imaging
摘要：The easily constructed fluorescent probes with excellent sensing performance have attracted increasing attention in recent years. In this work, by virtue of the contrasting light absorption properties of neutral red (NR) and its product NR-NO after the reaction with hypochlorous acid (HOCl) for the fluorescence released from 4-triphenyl-vinyl-biphenyl (TPEP)-based nanoparticles (T-NPs), a facile inner filter effect (IFE)-based ratiometric sensing platform (SP) was constructed. Upon the addition of HOCl, a typical ratiometric fluorescence change and weak cross-talk between the emission bands (128 nm <= Delta lambda(em) <= 182 nm) can be observed from SP, which effectively avoids the spectral overlap and ensures the high sensitivity (LOD = 62 nM) and accuracy in HOCl detection. In addition, because of the good water dispersibility and solubility featured in T-NPs and NR respectively, the SP can also be successfully applied to HOCl detection in 100% aqueous solution and live cell imaging. Moreover, the ratiometric SP avoids the complex covalent linking between fluorophore and energy acceptor, thereby providing great flexibility and convenience for its construction and application.