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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：中国化学会创始会士、常务理事，中国化工学会会士
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学西部校区知顺楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学西部校区知顺楼F-202 辽宁省大连市高新区凌工路2号，大连116024 课题组网址：http://peng-group.dlut.edu.cn/ E-mail: pengxj@dlut.edu.cn
电子邮箱：pengxj@dlut.edu.cn
Interaction of Ru(phen)(3)Cl-2 with graphene oxide and its application for DNA detection both in vitro and in vivo
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论文类型：期刊论文
发表时间：2013-09-07
发表刊物：JOURNAL OF MATERIALS CHEMISTRY B
收录刊物：EI、SCIE、Scopus
卷号：1
期号：33
页面范围：4146-4151
ISSN号：2050-750X
摘要：The fluorescence of Ru(phen)(3)Cl-2 (tris(1,10-phenanthroline) ruthenium(II) dichloride) can be effectively quenched with the addition of graphene oxide in aqueous solution, due to the formation of a GO-Ru hybrid. A fluorescence enhancement of approximately 50 times can be observed after the addition of a certain amount of DNA into the above-mentioned solution. The fluorescence increase is linearly proportional to the amount of DNA added in the concentration range of 0-70 mu M and the DNA detection limit is down to 3.3 x 10(-8) M. Meanwhile, it is found that the GO-Ru hybrid can enter into the nuclei and stain the DNA of living human breast cancer cells MCF-7, while Ru(phen)(3)Cl-2 alone cannot cross the cellular membrane in the control experiment. This method can be employed to detect DNA both in vitro and in vivo.