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个人信息Personal Information
教授
博士生导师
硕士生导师
主要任职：中国化学会创始会士、常务理事，中国化工学会会士
性别：男
毕业院校：大连理工大学
学位：博士
所在单位：化工学院
学科：应用化学. 精细化工. 化学生物学
办公地点：大连理工大学西部校区知顺楼F-202#
http://peng-group.dlut.edu.cn/
联系方式：大连理工大学西部校区知顺楼F-202 辽宁省大连市高新区凌工路2号，大连116024 课题组网址：http://peng-group.dlut.edu.cn/ E-mail: pengxj@dlut.edu.cn
电子邮箱：pengxj@dlut.edu.cn
Bright and stable Cy3-encapsulated fluorescent silica nanoparticles with a large Stokes shift
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论文类型：期刊论文
发表时间：2012-04-01
发表刊物：DYES AND PIGMENTS
收录刊物：SCIE、EI
卷号：93
期号：1-3
页面范围：1532-1537
ISSN号：0143-7208
关键字：Cyanine dyes; Fluorescence; HFRET; Silica nanoparticles; Stokes shift; Photostability
摘要：Traditional organic fluorophores, like cyanine dyes, suffer from their poor stability and weak brightness of individual molecule. In this work, a novel cyanine dye with a large Stokes shift ( similar to 75 nm) was encapsulated inside silica nanoparticles. The obtained small fluorescent silica nanoparticle (FSNP) exhibits more than ten times brightness than the free dye. The enhanced fluorescence brightness was assigned to the less homo Forster resonance energy transfer (HFRET) between multiple fluorophores, which was confirmed by the longer fluorescence lifetime of FSNP with a large Stokes shift than that with a normal Stokes shift. The FSNP's photostability is much better than organic fluorophores and comparable with that of Quantum Dots. When used in bioimaging, the FSNP remained a stable fluorescence signal, while the control free dye faded within 12 h. (C) 2011 Elsevier Ltd. All rights reserved.