He Gaohong   

Professor
Supervisor of Doctorate Candidates
Supervisor of Master's Candidates

Title : Director of R & D Center of Membrane Science and Technology

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Language:English

Paper Publications

Title of Paper:阳离子反胶团体系萃取牛血清白蛋白

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Date of Publication:2003-01-01

Journal:化工进展

Included Journals:PKU、ISTIC

Volume:22

Issue:z1

Page Number:206-210

ISSN No.:1000-6613

Key Words:牛血清白蛋白;反胶团体系;萃取率;萃取

Abstract:采用一种新的阳离子反胶团体系(CTAB/异辛烷-正戊醇),研究了反胶团萃取牛血清白蛋白(BSA).用单变量法考察了表面活性剂浓度、水相pH值、离子种类和浓度、萃取时搅拌速率和油水比对BSA萃取率的影响.实验表明,静电作用力是该萃取过程的主要动力,随水相pH值增大,BSA的萃取率逐渐升高;对于不同种类的离子,基本随其浓度增大,萃取率下降.在优化的操作条件下,BSA萃取率可达98%以上,因此,CTAB/异辛烷-正戊醇反胶团体系适合BSA的萃取.

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