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Indexed by:期刊论文

Date of Publication:2017-12-01

Journal:BIORESOURCE TECHNOLOGY

Included Journals:Scopus、SCIE、EI、PubMed

Volume:245

Issue:,SI

Page Number:1603-1609

ISSN No.:0960-8524

Key Words:L-Glutamine; L-Alanyl-L-glutamine; alpha-Amino acid ester acyltransferase; Sphingobacterium siyangensis; Repeated-cycle

Abstract:In this study, the optimum induction and reaction conditions, and fermentation process of producing Ala-Gln by E. coli Origami 2 overexpressing alpha-amino acid ester acyltransferase (OPA) were investigated. Besides, the Ala-Gln synthesis by OPA achieved the maximum molar yield of 94.7% and productivity of 1.89 g/L/min due to the extremely high enzyme activity. On this basis, repeated-cycle batch fermentation to produce Ala-Gln indicated that OPA could maintain high Ala-Gln yields and enzyme stabilities after several cell recycling. Consequently, the cost-efficient and environmentally friendly approach for Ala-Gln production by recycling OPA makes a great contribution to further industrial-scale applications. (C) 2017 Published by Elsevier Ltd.