Release Time:2019-03-11  Hits:

Indexed by: Journal Article

Date of Publication: 2017-12-01

Journal: BIORESOURCE TECHNOLOGY

Included Journals: PubMed、EI、SCIE、Scopus

Volume: 245

Issue: ,SI

Page Number: 1603-1609

ISSN: 0960-8524

Key Words: L-Glutamine; L-Alanyl-L-glutamine; alpha-Amino acid ester acyltransferase; Sphingobacterium siyangensis; Repeated-cycle

Abstract: In this study, the optimum induction and reaction conditions, and fermentation process of producing Ala-Gln by E. coli Origami 2 overexpressing alpha-amino acid ester acyltransferase (OPA) were investigated. Besides, the Ala-Gln synthesis by OPA achieved the maximum molar yield of 94.7% and productivity of 1.89 g/L/min due to the extremely high enzyme activity. On this basis, repeated-cycle batch fermentation to produce Ala-Gln indicated that OPA could maintain high Ala-Gln yields and enzyme stabilities after several cell recycling. Consequently, the cost-efficient and environmentally friendly approach for Ala-Gln production by recycling OPA makes a great contribution to further industrial-scale applications. (C) 2017 Published by Elsevier Ltd.