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Indexed by:期刊论文

Date of Publication:2017-02-17

Journal:JOURNAL OF BIOLOGICAL CHEMISTRY

Included Journals:SCIE、EI、PubMed

Volume:292

Issue:7

Page Number:2660-2669

ISSN No.:0021-9258

Key Words:Gcn2,Kog1,TOR complex (TORC),TORC1,amino acid,amino acid starvation,autophagy,histidine,uncharged tRNA,yeast

Abstract:In eukaryotic cells, two conserved protein kinases, Gcn2 and TOR complex 1 (TORC1), couple amino acid conditions to protein translation. Gcn2 functions as an amino acid sensor and is activated by uncharged tRNAs that accumulate when intracellular amino acids are limited. Activated Gcn2 phosphorylates and inhibits eukaryotic initiation factor-2 alpha (eIF2 alpha), resulting in repression of general protein synthesis. Like Gcn2, TORC1 is also involved in sensing amino acid conditions. However, the underlying mechanism remains unclear. In the present study, we show that TORC1 is a direct target of Gcn2 kinase in the yeast Saccharomyces cerevisiae. In response to amino acid starvation, Gcn2 binds to TORC1 and phosphorylates Kog1, the unique regulatory subunit of TORC1, resulting in down-regulation of TORC1 kinase activity. In the absence of Gcn2, TORC1 signaling activity increases and becomes unresponsive to amino acid starvation. Our findings demonstrate that TORC1 is an effector of Gcn2 in amino acid signaling, hence defining a novel mechanism by which TORC1 senses amino acid starvation.