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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：生物工程学院
学科：生物化工. 生物工程与技术. 药物工程
办公地点：生物楼512
联系方式：wangjingyun67@dlut.edu.cn
电子邮箱：wangjingyun67@dlut.edu.cn
A novel fluorescent sensor for detection of highly reactive oxygen species, and for imaging such endogenous hROS in the mitochondria of living cells
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论文类型：期刊论文
发表时间：2013-02-21
发表刊物：ANALYST
收录刊物：SCIE、PubMed、Scopus
卷号：138
期号：3
页面范围：775-778
ISSN号：0003-2654
摘要：A new dye, PTZ-Cy2, based on a hybrid cyanine-phenothiazine platform, is described. Oxidation by highly reactive oxygen species (hROS) involves attack at the thiazine sulfur atom and destruction of the pi conjugation of the cyanine moiety. Dual fluorescence emissions are enhanced dramatically at lambda(em) 470 nm and 595 nm, and PTZ-Cy2 thus serves both as an absorbance ratiometric and a fluorescent "off-on" sensor for detecting hROS. Moreover PTZ-Cy2 shows selectivity for hROS over other oxidants, and gives a highly sensitive response to such endogenous species within the mitochondria of living cells.