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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：生物工程学院
学科：生物化工. 生物工程与技术. 药物工程
办公地点：生物楼512
联系方式：wangjingyun67@dlut.edu.cn
电子邮箱：wangjingyun67@dlut.edu.cn
Spectroscopic Techniques Study on the Quenching Mechanism of Cytochrome B5 Affected by Cu(II)
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论文类型：期刊论文
发表时间：2013-04-01
发表刊物：ASIAN JOURNAL OF CHEMISTRY
收录刊物：SCIE、Scopus
卷号：25
期号：4
页面范围：2054-2058
ISSN号：0970-7077
关键字：Cytochrome B5; Quenching mechanism; Spectrosciopic techniques
摘要：The interaction between Cu2+ and cytochrome b5 (cytb5) has been investigated by multi-spectroscopic techniques. The results show that Cu2+ could interact with cytochrome b5 and induce fluorescence of the latter to be quenched. The quenching mechanism is dominated by dynamic quenching. Synchronous fluorescence and circular dichroism spectra demonstrate that Cu2+ could also influence the microcircumatance of aromatic amino residues and secondary structure of the protein.