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个人信息Personal Information
教授
博士生导师
硕士生导师
性别：女
毕业院校：大连理工大学
学位：博士
所在单位：生物工程学院
学科：生物化工. 生物工程与技术. 药物工程
办公地点：生物楼512
联系方式：wangjingyun67@dlut.edu.cn
电子邮箱：wangjingyun67@dlut.edu.cn
Zn2+ Binding-Enabled Excited State Intramolecular Proton Transfer: A Step toward New Near-Infrared Fluorescent Probes for Imaging Applications
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论文类型：期刊论文
发表时间：2012-07-01
发表刊物：ADVANCED HEALTHCARE MATERIALS
收录刊物：SCIE、Scopus
卷号：1
期号：4
页面范围：485-492
ISSN号：2192-2640
摘要：In order to facilitate the in vivo study of zinc-related biology, it is essential to develop a zinc-selective sensor that exhibits both near-infrared (NIR) emission and larger Stokes shift. A fluorescent sensor, Zinhbo-5, has been constructed by using bis(benzoxazole) ligand with 2,2'-dipicolylamine (DPA) as metal ion receptor. In aqueous solution, Zinhbo-5 exhibits high sensitivity (K-d = 2.58 nM(2)) and selectivity for Zn2+ cation, revealing about 14-fold fluorescence enhancement upon zinc binding to give green emission. Remarkably, Zn2+ binding to Zinhbo-5 switches on the excited state intramolecular proton transfer (ESIPT), producing the desirable near-infrared region (over 710 nm) with large Stokes shift (ca. 240 nm). The new probe is demonstrated to be useful for in vivo imaging of the intracellular Zn2+ ion. The Zinhbo-5 is also useful for detecting zinc ion distribution during the development of living zebrafish embryos.