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Indexed by:期刊论文

Date of Publication:2014-07-01

Journal:JOURNAL OF FLUORESCENCE

Included Journals:SCIE、EI、Scopus

Volume:24

Issue:4

Page Number:1313-1317

ISSN No.:1053-0509

Key Words:Inorganic mercury; Neuron; Fluorescence probe

Abstract:In the present study, we used the previously developed fluorescence probe, EPNP, to generate the first image of the distribution of mercuric ion in primary mouse neuron cultures. At postnatal day 1 (P1), the mice were intraperitoneally (IP) injected with mercuric chloride in doses ranging from 0.05 to 0.6 mu g/g body weight. After 1, 2, 3, and 4 days exposure, primary nervous cell cultures and frozen brain and spinal tissue sections were prepared and dyed using EPNP. On the third day of repeated injections, Hg2+ was visualized in primary cerebral neuron cultures as an increase of Hg2+-induced fluorescence at the doses a parts per thousand yenaEuro parts per thousand 0.1 mu g/g. A similar accumulation of Hg2+ was observed in frozen hippocampus tissue sections. In contrast, no Hg2+ was observed in spinal cord neurons and spinal tissue sections. The detection of a low dose of IP injected mercury in mouse cerebral neurons facilitated the evaluation of the exposure risk to low-dose Hg2+ in immature organisms. Moreover, the highly sensitive EPNP revealed Hg2+ in the cerebral neurons of mice younger than P4, while the presence of Hg2+ was not detected until a parts per thousand yenaEuro parts per thousand P11 in previous reports. Thus, this technology and the results obtained herein are of interest for neurotoxicology.