Release Time:2019-03-09  Hits:

Indexed by: Journal Article

Date of Publication: 2014-07-01

Journal: JOURNAL OF FLUORESCENCE

Included Journals: Scopus、EI、SCIE

Volume: 24

Issue: 4

Page Number: 1313-1317

ISSN: 1053-0509

Key Words: Inorganic mercury; Neuron; Fluorescence probe

Abstract: In the present study, we used the previously developed fluorescence probe, EPNP, to generate the first image of the distribution of mercuric ion in primary mouse neuron cultures. At postnatal day 1 (P1), the mice were intraperitoneally (IP) injected with mercuric chloride in doses ranging from 0.05 to 0.6 mu g/g body weight. After 1, 2, 3, and 4 days exposure, primary nervous cell cultures and frozen brain and spinal tissue sections were prepared and dyed using EPNP. On the third day of repeated injections, Hg2+ was visualized in primary cerebral neuron cultures as an increase of Hg2+-induced fluorescence at the doses a parts per thousand yenaEuro parts per thousand 0.1 mu g/g. A similar accumulation of Hg2+ was observed in frozen hippocampus tissue sections. In contrast, no Hg2+ was observed in spinal cord neurons and spinal tissue sections. The detection of a low dose of IP injected mercury in mouse cerebral neurons facilitated the evaluation of the exposure risk to low-dose Hg2+ in immature organisms. Moreover, the highly sensitive EPNP revealed Hg2+ in the cerebral neurons of mice younger than P4, while the presence of Hg2+ was not detected until a parts per thousand yenaEuro parts per thousand P11 in previous reports. Thus, this technology and the results obtained herein are of interest for neurotoxicology.