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Indexed by:期刊论文

Date of Publication:2017-02-01

Journal:FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT

Included Journals:SCIE、EI、PubMed、Scopus

Volume:34

Issue:2

Page Number:255-264

ISSN No.:1944-0049

Key Words:Mycotoxins; vegetable oil; QuEChERS; HPLC-MS; MS

Abstract:A simple and efficient method for determining multiple mycotoxins was developed using a QuEChERS (quick, easy, cheap, effective, rugged and safe)-based extraction procedure in vegetable oils. High-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used for the quantification and confirmation of 16 chemically diversified mycotoxins. Different extraction procedures were studied and optimised by spiking 16 analytes into blank matrix, and the extraction with 85% MeCN solution and C18 as cleaning sorbent allowed an efficient recovery of 72.8-105.8% with RSDs less than 7%. The limit of detection (LOD) ranged from 0.04 to 2.9 ngg(-1). The developed method was finally applied to screen mycotoxins in 62 vegetable oil samples. Zearalenone (ZEN), aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and -zearalenol (-ZOL) were detected, with maximum concentrations of 0.59 (AFG1)-42.5 (ZEN) ngg(-1). The method developed has the advantages of high sensitivity, accuracy and selectivity, and it can be applied to the target screening of mycotoxins in real samples.