Release Time:2020-02-17  Hits:

Indexed by: Journal Papers

Date of Publication: 2019-11-01

Journal: BIOINTERPHASES

Included Journals: SCIE、PubMed

Volume: 14

Issue: 6

Page Number: 061003

ISSN: 1934-8630

Abstract: The reliability and stability of array slides are a big concern for array vendors and end users. Herein, we report on a new type of array slide with high reactivity toward DNA probes and low side reaction. A one-step surface reaction via the Michael addition involved in preparing array slides was developed and characterized by x-ray photoelectron spectroscopy, contact angle, and fluorescence labeling. The effects of array fabrication and storage conditions, i.e., spotting solution pH, high humidity, and long-term storage on the reactivity of the slides were examined. The fabricated DNA arrays could realize good hybridization efficiency (38.2% for slides with 0.88 pmol/cm(2)), low limit of detection (4 x 10(-14)M), as well as high mismatch selectivity.