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Indexed by:Journal Papers

Date of Publication:2019-11-01

Journal:BIOINTERPHASES

Included Journals:PubMed、SCIE

Volume:14

Issue:6

Page Number:061003

ISSN No.:1934-8630

Abstract:The reliability and stability of array slides are a big concern for array vendors and end users. Herein, we report on a new type of array slide with high reactivity toward DNA probes and low side reaction. A one-step surface reaction via the Michael addition involved in preparing array slides was developed and characterized by x-ray photoelectron spectroscopy, contact angle, and fluorescence labeling. The effects of array fabrication and storage conditions, i.e., spotting solution pH, high humidity, and long-term storage on the reactivity of the slides were examined. The fabricated DNA arrays could realize good hybridization efficiency (38.2% for slides with 0.88 pmol/cm(2)), low limit of detection (4 x 10(-14)M), as well as high mismatch selectivity.