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Date of Publication:2017-01-01

Journal:物理化学学报

Affiliation of Author(s):化工学院

Volume:33

Issue:2

Page Number:426-434

ISSN No.:1000-6818

Abstract:Carbohydrates chips are powerful tools for quantitatively studying protein-carbohydrate interactions.Typically,carbohydrate chips are prepared using the self-assembly of carbohydrate thiol/disulfide,which always requires multiple hydroxyl group protection/deprotection steps resulting low conversion in the preparation.In this paper,a kind of carbohydrate derivatives containing dithiocarbamate (DTC) group was synthesized through a two-step reaction to prepare self-assembled monolayers presenting carbohydrate (glycol-DTC SAMs).The glycol-DTC SAMs was characterized using X-ray photoelectron spectroscopy (XPS) and the protein binding activity was quantitatively analysized using surface plasma resonance (SPR) and enzyme linked lectin assay (ELLA).By mixed self-assembly of carbohydrate dithiocarbamate and sarcosine dithiocarbamate,Glycol-DTC SAMs with different glucose density were prepared.The protein binding kinetics was monitored in real time and the thermodynamics was calculated.Interestingly,a 1:1 binding of concanavalin A (Con A) was obtained on the SAMs prepared in solution containing 1％ glucose-DTC,as the dissociation constant (Kd) was calculated to be (39.10 ± 0.12) μmol· L-1.A 1:2 binding of Con A was obtained on the SAMs prepared in solutions containing ＞ 2％ glucose-DTC,as the Kd was calculated to be (1.17 ± 0.18) μmol·L-1.By simply mixed self assembly,multivalent binding of Con A can be realized and separate kinetic parameters can be obtained.Our work would promote the study of protein-carbohydrate interactions and be helpful for revealing the relevant biological progress.

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