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Indexed by:期刊论文

Date of Publication:2022-06-28

Journal:物理化学学报

Affiliation of Author(s):化工学院

Volume:34

Issue:2

Page Number:177-184

ISSN No.:1000-6818

Abstract:Control over the ligand surface density provides an accurate molecular
   basis for the quantitative study of biomolecular interactions. However,
   the classic hybrid self-assembly method lacks general applicability
   toward different self-assembly systems. In this paper, we report a new
   method based on the reaction kinetics of vinyl sulfone groups presented
   on surface to control the surface ligand density.
   Na,Na-bis(carboxymethyl)-L-lysine (ab-NTA) was selected as the model
   biological ligand and the catalyst for surface reaction was screened.
   The surface reaction was characterized by X-ray photoelectron
   spectroscopy (XPS) and the surface membrane potential. Static water
   contact angle was used to quantify the kinetics of the surface reaction,
   and calculations showed that the rate constant was 0.0012 min~(-1). The
   ability of the biological functional surface to bind a histidine
   labeling protein (SA-6His) was investigated by surface plasmon resonance
   (SPR). The results show that such a surface has a higher protein binding
   quantity and binding strength than the traditional NHS-NTA surface. Four
   biological functional surfaces with different ligand densities were
   prepared by controlling the reaction time and catalyst, and the protein
   static adsorption of these surfaces was analyzed by SPR. The results
   show that ligand density and multivalence of the biological functional
   surface can be controlled by modulating the reaction time and catalyst.

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