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发布时间：2019-03-13

论文类型：期刊论文

发表时间：2016-07-01

发表刊物：INTERNATIONAL IMMUNOPHARMACOLOGY

收录刊物：Scopus、PubMed、SCIE

卷号：36

页面范围：305-314

ISSN号：1567-5769

关键字：Egg yolk antibodies; IgY; Salmonella typhimurium; Mucosal immune response; Intestinal inflammation; Gut-associated lymphoid tissue

摘要：This study determined the effects of chicken egg yolk antibodies (IgY) on immune responses in the intestinal mucosal of mice infected with Salmonella typhimurium. Sixty, 28-day-old mice were divided into 4 groups and treated with streptomycin or sterile water for 2 days followed by 1 day without treatment. The control group was unchallenged whereas the mice in the other three groups were treated twice with 10(9) CFU mL(-1) S. typhimurium. For the next 3 days, control mice continued to receive no treatment whereas the mice in the remaining three groups were orally administered with 20 mg mL(-1) of specific IgY, 20 mg mL(-1) of nonspecific IgY or PBS. S. typhimurium activated gut-associated lymphoid tissue, increasing the release of IFN-gamma and TNF-alpha in the mucosa and increased the number of activated T-lymphocytes and cytotoxic T-gamma delta. Specific IgY attenuated the increase in IFN-gamma and TNF-alpha and the decrease in IL-10. S. typhimurium induced mobilization of CD8(+) and CD8(+) TCR gamma delta T cells in the epithelium and CD4(+) and CD8(+) T cells in the lamina propria reflecting an inflammatory process that was attenuated by IgY. These results suggest that specific IgY modulates intestinal mucosal immune responses during a S. typhimurium infection. (C) 2016 Elsevier B.V. All rights reserved.